Altered Circadian Rhythm of Melatonin Secretion and Circadian Gene Expression in the Early Stage of Sepsis

In sepsis patients, do melatonin secretion and circadian gene expression compared to non-septic patients alter from their normal rhythm?

Status

Completed

Phases

Unknown

Study type

Observational

Source

ANZCTR

Registry ID

ACTRN12612000494831

Enrollment

Registered

2012-05-07

Start date

2011-09-01

Completion date

Unknown

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

This study will investigate the melatonin secretion and its rhythm in septic patients in ICU compared to that in non-septic patients, as well as circadian gene expression and TNF-a and IL-6 level.

Interventions

Eleven septic patients critical illness patients were enrolled in this prospective study. Peripheral blood was drawn every four hours from 2 p.m. on their first ICU day up to 24 hrs. Plasma levels of melatonin, tumor necrosis factor (TNF)-a and interleukin (IL)-6 were measured by radioimmunoassay or enzyme-linked immunosorbent assay. Message RNA levels of circadian genes, Cry-1 and Per-2, were analyzed using quantitative real-time polymerase chain reaction.

Eligibility

Sex/Gender

All

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

critically ill patients (both sepsis and non-sepsis patients) admitted to the studied ICU in a university hospital

Exclusion criteria

1. Patients with insomnia or mental illness; 2. Patients who had shift work before admission to the hospital; 3. Patients who received ß-blockers, melatonin or blocking agents of melatonin.

Outcome results