Exercise and postprandial lipemia - The effect of exercise and bioactive nutrients on vascular and metabolic response to postprandial lipemia

The study will consist of three sessions; each session will be separated by a one week wash-out period. In Session 1, subjects will consume a high fat meal (HFM). The fat-rich meal will be in milkshake form and will consist of 270 ml of whipping cream and 65 g of specialty ice cream. The meal will provide 4.1 MJ (980 kcal; 0.05 MJ/kg body weight), 100 g fat (1.1 g/kg); 66.5 g saturated, 29.5 monounsaturated, 4.0 g polyunsaturated, 17 g carbohydrate (0.2 g/kg), and 3 g protein (0.03 g/kg). This meal has been successfully used in previous studies to induce postprandial lipemia. All measures will be assessed at pre and post consumption of a HFM. In Session 2, a combination of a HFM and green tea capsules (GTC) will be consumed by subjects; all measures then will be assessed at pre and post consumption of a HFM and GTC combined. The green tea extract will be in capsule form (3 capsules), which will contain 375 mg of epigallo catechin gallate (EGCG). In Session 3, subjects will consume a HFM and GTC, then 2 hours post consumption of a HFM and GTC subjects will undergo a single bout of exercise. All measures will be assessed at pre and post consumption of a HFM and GTC capsules and post acute exercise. The acute exercise protocol will consist of 30 min of steady state exercise on a stationary Monark bike at 60% of subject’s VO2peak. During exercise, subjects will be supervised and their heart rate will be monitored every 2 min by the tester/researcher.