DNA damage and Nutritional Profile of ApoE4 carriers, the Major Genetic Risk Factor for Alzheimer’s Disease

DNA Damage In Cells of Human ApoE4 Carriers and Prevention In vitro By Nutritional Supplementation with Lipophilic Antioxidants and Fatty Acids

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

ANZCTR

Registry ID

ACTRN12612000098831

Enrollment

200

Registered

2012-01-20

Start date

2011-12-10

Completion date

Unknown

Last updated

2020-01-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

None listed

Brief summary

Alzheimer’s disease (AD) is the most common form of dementia, currently affecting at least 35 million people worldwide and expected to double every 20 years. The risk of acquiring Alzheimer’s doubles every five years after the age of 65 and may be affected by common alterations in the fat transporter gene (APOE). The brain contains large proportions of fats such as cholesterol and omega-3 fatty acids. APOE plays a crucial role in maintaining healthy fat distribution such as cholesterol and omega-3 fatty acids, as well as brain cell repair and scavenging of toxins. Common alterations in the APOE gene, may directly or indirectly increase DNA damage and accelerate brain aging and degeneration. This disease is currently considered incurable and greatly highlights the need for preventative measures and therapeutic interventions, such as nutritional supplementation, that may improve DNA stability and mental health.

Interventions

This study aims to determine the DNA damage, lipid and micronutrient profile of ApoE4 carriers compared to non-carriers, and to determine if DNA damage in lymphocytes of ApoE4 carriers can be prevented with nutritional supplementation. A single fasted blood and cheek cell sample will be collected to determine DNA, micronutrient and fat content status, which may be associated with Alzheimer's disease. Dietary and lifestyle questionnaires and a short cognitive assessment will also be administered to measure mineral and vitamin intake and assess any lifestyle activities that may impact upon Alzheimer disease and dementia risk. All samples will be collected and questionnaires performed during the single visit. No dietary or drug treatment is involved. Recruitment will occur over approximately 5-6 months or until target sample size has been reached.

Eligibility

Sex/Gender

All

Age

35 Years to 65 Years

Healthy volunteers

Yes

Inclusion criteria

Inclusion Criteria Aged 35-65 years; Healthy; Non-smokers; Male & Female

Exclusion criteria

Exclusion Criteria: Mini-mental State Examination (MMSE) score of less than 20. (short mental examination); Currently diagnosed with AD or Mild cognitive impairment; On Medication for life threatening diseases (i.e. chemotherapy); Taking mineral and vitamin supplements above the RDA level on a daily basis; Taking fish oil and antioxidant supplements investigated in the study; Unable to understand the study protocol

Outcome results