None listed
Conditions
Brief summary
The reponses of the human body to a high fat meal are largely unknown. This study aims to examine the responses of the immune system, fat cells and muscle cells to a breakfast meal containing saturated fats or polyunsaturated fats. This study will specifically investigate the saturated fat that comes fom dairy foods, including milk, butter, yoghurt and cheese. Our hypothesis is that although dairy is rich in saturated fat, many of these fats are beneficial for human health.
Interventions
Male participants (aged 40-60 years) will randomise to ingest differing breakfasts. Four possible breakfast meals (treatment arms) will be provided. These include; Arm 1: High fat dairy breakfast. Arm 2: High fat non-dairy breakfast. Arm 3: Low fat dairy brekfast. Arm 4: Low fat non-dairy breakfast. Subjects will be randomised to ingest either arms 1 and 2 or 3 and 4, with sequence of each meal being further randomly allocated.
Arms 1 and 2 are equal in total energy (3120kJ Arm 1 versus 3250k
Male participants (aged 40-60 years) will randomise to ingest differing breakfasts. Four possible breakfast meals (treatment arms) will be provided. These include; Arm 1: High fat dairy breakfast. Arm 2: High fat non-dairy breakfast. Arm 3: Low fat dairy brekfast. Arm 4: Low fat non-dairy breakfast. Subjects will be randomised to ingest either arms 1 and 2 or 3 and 4, with sequence of each meal being further randomly allocated. Arms 1 and 2 are equal in total energy (3120kJ Arm 1 versus 3250kJ Arm 2), and equal for macronutrients (54g fat, 20g protein and 37g carbohydrate). Arms 3 and 4 are equal in total energy (1860kJ Arm 3 versus 2010kJ Arm 4), and equal for macronutrients (19g fat, 29g protein and 39g carbohydrate). Subjects will arrive at the laboratory in a fasted stated. Breakfast will be supplied and eaten within 15 minutes. Following each breakfast, blood samples, as well as adipose and muscle samples (via biopsy) will be taken. These will be used to determine what biological and metabolic responses occur after food ingestion. Breakfasts meals will be separated by a 4 week wash-out period.
Sponsors
Deakin University
Study design
Allocation
Randomised controlled trial
Intervention model
Parallel
Primary purpose
Prevention
Masking
Blinded (masking used)
Eligibility
Sex/Gender
Male
Age
40 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
Lean subjects are included based on a waist circumference less than 94cm. Metabolic Syndrome subjects are included based on having a waist circumference greater than 94cm, plus two of the following; fasting serum triglycerides greater or equal to 1.7mmol/l, fasting plasma glucose greater or equal to 5.6mmol/l; serum high density lipoprotein cholesterol (HDL) less than 1.03mg/l; systolic blood pressure either greater than or equal to systolic 130mmHG.
Exclusion criteria
The presence of confirmed diabetes or heart disease.
Outcome results
None listed