None listed
Conditions
Brief summary
This study aims at verifying the effect of filtered coffee intake on oxidative stress and inflammatory biomarkers in health volunteers and quantify phenolic acids in the coffee brew and in the human plasma.
Interventions
Twenty health volunteers, with age between 20 and 65 years old, will be selected and undergo 28 days of washout before the start the treatment. Then they will be divided in two groups: one will ingest from 3 to 4 cups daily (100 mL each one for people over 60 years old and 150 mL for people under 60 years old) of coffee brew with light roasting and another with dark roasting for 4 weeks. After, will be made a cross-over and the volunteers will ingest the coffee brew for over 4 weeks. Coffee will
Twenty health volunteers, with age between 20 and 65 years old, will be selected and undergo 28 days of washout before the start the treatment. Then they will be divided in two groups: one will ingest from 3 to 4 cups daily (100 mL each one for people over 60 years old and 150 mL for people under 60 years old) of coffee brew with light roasting and another with dark roasting for 4 weeks. After, will be made a cross-over and the volunteers will ingest the coffee brew for over 4 weeks. Coffee will be provided to participants in daily portion. There will not have a washout between the two coffee groups, because studies show that phenolic acids bioavailability is short (After 4 hours of coffee intake, phenolic acid are not present in plasma in detectable amount), therefore 4 weeks are sufficient to eliminate the effect of one rosting on the other).
Sponsors
University of Sao Paulo (USP) - School of Public Health
Study design
Allocation
Randomised controlled trial
Intervention model
Crossover
Primary purpose
Prevention
Masking
Blinded (masking used)
Eligibility
Sex/Gender
All
Age
20 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
All races, non-smokers or former-smokers for more than 2 years, non-alcoholics, serum cholesterol below 240 mg/dL, fasting glucose below 100 mg/dL, normal liver enzymes (Aspartate aminotransferase - AST and Alanine aminotransferase - ALT) and normal plasma homocysteine, absence of continuous medicine intake. No history of cardivascular, kidney or liver disease, diabetes mellitus, hypertension or hyperthyroidism.
Exclusion criteria
Smoker or former-smoker for less than 2 years, alcoholic, serum cholesterol above 240 mg/dL, fasting glucose above 100mg/dL, altered liver enzymes, and normal plasma homocysteine; medication intake daily; presence or development of cardiovascular, kidney or liver disease, diabetes mellitus, hypertension or hyperthyroidism.
Outcome results
None listed