Observational Study and follow up of a Sepsis Biomarker for Sepsis Diagnosis in the Intensive Care

Baseline blood samples will be taken from all patients enrolled. Theses samples include electrolytes, arterial blood gas, complete blood count, C-reactive protein, lactate, Interluken-6 (IL-6), procalcitonin and oxidised protein sepsis markers. Patients who are suspected to have sepsis (given antibiotics) and/or has systemic inflammatory response score (SIRS)>=3 will have complete sepsis biomarkers blood tests. The complete sepsis biomarkers blood tests consist of blood samples taken at T=0 (time of onset of sickness), T=12 hrs, T=24 hrs, T=48 hrs and time of recovery (no sooner than T=72 hrs). At T=0, blood samples are taken for bacterial cultural if suspected for sepsis, electrolytes, arterial blood gas, complete blood count, prothrombin ration, activated partial thromboplastin time (APTT), fibrinogen, C-reactive protein, lactate, Interluken-6 (IL-6), procalcitonin and oxidised protein sepsis markers. In addition, during the first hour, blood samples for insulin and c-peptide will be taken at T=0, 10, 40 and 60 mins. At T=12 hrs, blood samples are taken for electrolytes, arterial blood gas, complete blood count, C-reactive protein, lactate, Interluken-6 (IL-6), procalcitonin and oxidised protein sepsis markers. At T=24 hrs, blood samples are taken for bacterial cultural if suspected for sepsis, electrolytes, arterial blood gas, complete blood count, C-reactive protein, lactate, Interluken-6 (IL-6), procalcitonin and oxidised protein sepsis markers. At T=48 hrs, blood samples are taken for electrolytes, arterial blood gas, complete blood count, C-reactive protein, Interluken-6 (IL-6), procalcitonin and oxidised protein sepsis markers. Once patient recovers, defined as SIRS<=2 for 6 hours after T=72 hrs or transferring out of ICU in <=12 hours, blood samples are taken for electrolytes, arterial blood gas, complete blood count, C-reactive protein, Interluken-6 (IL-6), procalcitonin and oxidised protein sepsis markers. In addition, blood samples for insulin and c-peptide will be taken at T=0, 10, 40 and 60 mins from time of recovery.